Your browser doesn't support javascript.
loading
Effects of Prunus Tomentosa Thumb Total Flavones on adjuvant arthritis in rats and regulation of autophagy.
Chen, Xi; Sun, Lijuan; Wang, Shuang; Wang, Yilin; Zhou, Yue; Li, Yan; Cheng, Zihao; Wang, Yingying; Jiang, Yanan; Zhao, Zihan; Xv, Yawei; Zhang, Chengyi.
Afiliação
  • Chen X; College of Basic Medicine, Beihua University, Jilin, China.
  • Sun L; College of Basic Medicine, Beihua University, Jilin, China.
  • Wang S; College of Basic Medicine, Beihua University, Jilin, China.
  • Wang Y; State Key Laboratory of Quality Research in Chinese Medicine, Macao University of Science and Technology, Macao, China.
  • Zhou Y; Famous Doctor Studio of Zhuhai Integrated Traditional Chinese and Western Medicine Hospital, Zhuhai, Guangdong, China.
  • Li Y; Mingzheng Forensic Identification Centre of Jilin, Jilin, China.
  • Cheng Z; Food and Drug Inspection Institute of Jilin City, Jilin, China.
  • Wang Y; College of Pharmacy, Beihua University, Jilin, China.
  • Jiang Y; College of Basic Medicine, Beihua University, Jilin, China.
  • Zhao Z; College of Basic Medicine, Beihua University, Jilin, China.
  • Xv Y; College of Pharmacy, Beihua University, Jilin, China.
  • Zhang C; College of Pharmacy, Beihua University, Jilin, China.
Technol Health Care ; 31(S1): 123-136, 2023.
Article em En | MEDLINE | ID: mdl-37038787
BACKGROUND: Rheumatoid arthritis (RA) is a slow in taking effect systemic autoimmune disease. Prunus Tomentosa Thumb Total Flavones (PTTTF) has anti-inflammatory and antioxidant properties. OBJECTIVE: The purpose of this study is to the PTTTF on adjuvant arthritis (AA) in rats and to explore the mechanism of autophagy. METHODS: Adjuvant arthritis model was established in rats. The cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), interleukin-1ß (IL-1ß), interleukin-6 (IL-6), interleukin-17 (IL-17), tumor necrosis factor (TNF-α) of rat synovial tissue were determined by RT-PCR. The histopathological varieties of knee joints in AA rats were observed by HE staining. The expressions of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2 in rat synovial tissue were determined by Western Blotting. RESULTS: PTTTF (50, 100, 200 mg/kg) significantly inhibited inflammation in rats (P< 0.01). PTTTF significantly inhibited inflammatory factor COX in rat synovial tissue. COX-2, IL-1ß, IL-6, IL-17, TNF-α expression (P< 0.05); PTTTF can significantly improve the pathological damage of rat knee joint PTTTF and can significantly inhibited the expression of autophagy-related proteins in rat synovium (P< 0.05 ). CONCLUSION: PTTTF can inhibit adjuvant arthritis in rats and can inhibit the expression of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Experimental Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Technol Health Care Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite Experimental Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Technol Health Care Ano de publicação: 2023 Tipo de documento: Article