Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome.
Chem Commun (Camb)
; 59(34): 5106-5109, 2023 Apr 25.
Article
em En
| MEDLINE
| ID: mdl-37039083
ABSTRACT
A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation of a cleavable linker via the click reaction, achieving 8-oxoGua pull-down and sequencing. Using APSC-8-oxoGua-seq, 8-oxoGua can be identified at single-base resolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
Idioma:
En
Revista:
Chem Commun (Camb)
Ano de publicação:
2023
Tipo de documento:
Article