Somatic Cell Nuclear Transfer in Pigs.

Glanzner, Werner G; Rissi, Vitor B; Bordignon, Vilceu

Glanzner, Werner G; Rissi, Vitor B; Bordignon, Vilceu.

Afiliação

Glanzner WG; Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Rissi VB; Faculty of Veterinary Medicine, Federal University of Santa Catarina, UFSC, Curitibanos, SC, Brazil.
Bordignon V; Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada. vilceu.bordignon@mcgill.ca.

Methods Mol Biol ; 2647: 197-210, 2023.

Article em En | MEDLINE | ID: mdl-37041336

RESUMO

Somatic cell nuclear transfer (SCNT) has been successfully applied to clone animals of several species. Pigs are one of the main livestock species for food production and are also important for biomedical research due to their physiopathological similarities with humans. In the past 20 years, clones of several swine breeds have been produced for a variety of purposes, including biomedical and agricultural applications. In this chapter, we describe a protocol to produce cloned pigs by SCNT.

Assuntos

Clonagem de Organismos; Técnicas de Transferência Nuclear; Suínos; Animais; Humanos; Clonagem de Organismos/métodos

Palavras-chave

Embryo transfer; Enucleation; Histone deacetylases; Nuclear reprogramming; Nuclear transplantation; SCNT; Sus scrofa; Swine; Transcriptional inhibition

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clonagem de Organismos / Técnicas de Transferência Nuclear Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2023 Tipo de documento: Article

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