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Generation and Functional Analysis of Defective Viral Genomes during SARS-CoV-2 Infection.
Zhou, Terry; Gilliam, Nora J; Li, Sizhen; Spandau, Simone; Osborn, Raven M; Connor, Sarah; Anderson, Christopher S; Mariani, Thomas J; Thakar, Juilee; Dewhurst, Stephen; Mathews, David H; Huang, Liang; Sun, Yan.
Afiliação
  • Zhou T; Department of Immunology and Microbiology, University of Rochester Medical Center, Rochester, New York, USA.
  • Gilliam NJ; Department of Immunology and Microbiology, University of Rochester Medical Center, Rochester, New York, USA.
  • Li S; Medical Scientist Training Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
  • Spandau S; Translational Biomedical Sciences PhD Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
  • Osborn RM; School of Electrical Engineering & Computer Science, Oregon State University, Corvallis, Oregon, USA.
  • Connor S; Department of Immunology and Microbiology, University of Rochester Medical Center, Rochester, New York, USA.
  • Anderson CS; Department of Immunology and Microbiology, University of Rochester Medical Center, Rochester, New York, USA.
  • Mariani TJ; Translational Biomedical Sciences PhD Program, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
  • Thakar J; Department of Pediatrics and Center for Children's Health Research, University of Rochester, Rochester, New York, USA.
  • Dewhurst S; Department of Pediatrics and Center for Children's Health Research, University of Rochester, Rochester, New York, USA.
  • Mathews DH; Department of Pediatrics and Center for Children's Health Research, University of Rochester, Rochester, New York, USA.
  • Huang L; Department of Immunology and Microbiology, University of Rochester Medical Center, Rochester, New York, USA.
  • Sun Y; Department of Biostatistics and Computational Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA.
mBio ; 14(3): e0025023, 2023 06 27.
Article em En | MEDLINE | ID: mdl-37074178
Defective viral genomes (DVGs) have been identified in many RNA viruses as a major factor influencing antiviral immune response and viral pathogenesis. However, the generation and function of DVGs in SARS-CoV-2 infection are less known. In this study, we elucidated DVG generation in SARS-CoV-2 and its relationship with host antiviral immune response. We observed DVGs ubiquitously from transcriptome sequencing (RNA-seq) data sets of in vitro infections and autopsy lung tissues of COVID-19 patients. Four genomic hot spots were identified for DVG recombination, and RNA secondary structures were suggested to mediate DVG formation. Functionally, bulk and single-cell RNA-seq analysis indicated the interferon (IFN) stimulation of SARS-CoV-2 DVGs. We further applied our criteria to the next-generation sequencing (NGS) data set from a published cohort study and observed a significantly higher amount and frequency of DVG in symptomatic patients than those in asymptomatic patients. Finally, we observed exceptionally diverse DVG populations in one immunosuppressive patient up to 140 days after the first positive test of COVID-19, suggesting for the first time an association between DVGs and persistent viral infections in SARS-CoV-2. Together, our findings strongly suggest a critical role of DVGs in modulating host IFN responses and symptom development, calling for further inquiry into the mechanisms of DVG generation and into how DVGs modulate host responses and infection outcome during SARS-CoV-2 infection. IMPORTANCE Defective viral genomes (DVGs) are generated ubiquitously in many RNA viruses, including SARS-CoV-2. Their interference activity to full-length viruses and IFN stimulation provide the potential for them to be used in novel antiviral therapies and vaccine development. SARS-CoV-2 DVGs are generated through the recombination of two discontinuous genomic fragments by viral polymerase complex, and this recombination is also one of the major mechanisms for the emergence of new coronaviruses. Focusing on the generation and function of SARS-CoV-2 DVGs, these studies identify new hot spots for nonhomologous recombination and strongly suggest that the secondary structures within viral genomes mediate the recombination. Furthermore, these studies provide the first evidence for IFN stimulation activity of de novo DVGs during natural SARS-CoV-2 infection. These findings set up the foundation for further mechanism studies of SARS-CoV-2 recombination and provide evidence to harness the immunostimulatory potential of DVGs in the development of a vaccine and antivirals for SARS-CoV-2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus de RNA / COVID-19 Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: MBio Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus de RNA / COVID-19 Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: MBio Ano de publicação: 2023 Tipo de documento: Article