Characterization of a TetR-type positive regulator AtrA for lincomycin production in Streptomyces lincolnensis.
Biosci Biotechnol Biochem
; 87(7): 786-795, 2023 Jun 23.
Article
em En
| MEDLINE
| ID: mdl-37076767
AtrA belongs to the TetR family and has been well characterized for its roles in antibiotic biosynthesis regulation. Here, we identified an AtrA homolog (AtrA-lin) in Streptomyces lincolnensis. Disruption of atrA-lin resulted in reduced lincomycin production, whereas the complement restored the lincomycin production level to that of the wild-type. In addition, atrA-lin disruption did not affect cell growth and morphological differentiation. Furthermore, atrA-lin disruption hindered the transcription of regulatory gene lmbU, structural genes lmbA and lmbW inside the lincomycin biosynthesis gene cluster, and 2 other regulatory genes, adpA and bldA. Completement of atrA-lin restored the transcription of these genes to varying degrees. Notably, we found that AtrA-lin directly binds to the promoter region of lmbU. Collectively, AtrA-lin positively modulated lincomycin production via both pathway-specific and global regulators. This study offers further insights into the functional diversity of AtrA homologs and the mechanism of lincomycin biosynthesis regulation.
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01-internacional
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MEDLINE
Assunto principal:
Streptomyces
/
Lincomicina
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Ano de publicação:
2023
Tipo de documento:
Article