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Sequestration of cucurbitacins from cucumber plants by Diabrotica balteata larvae provides little protection against biological control agents.
Bruno, Pamela; Arce, Carla C M; Machado, Ricardo A R; Besomi, Gaia; Spescha, Anna; Glauser, Gaétan; Jaccard, Charlyne; Benrey, Betty; Turlings, Ted C J.
Afiliação
  • Bruno P; Laboratory of Fundamental and Applied Research in Chemical Ecology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
  • Arce CCM; Laboratory of Fundamental and Applied Research in Chemical Ecology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
  • Machado RAR; Experimental Biology Group, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
  • Besomi G; Laboratory of Fundamental and Applied Research in Chemical Ecology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
  • Spescha A; Plant Pathology Group, Institute of Integrative Biology, ETH Zürich, Zürich, Switzerland.
  • Glauser G; Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, Neuchâtel, Switzerland.
  • Jaccard C; Laboratory of Evolutionary Entomology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
  • Benrey B; Laboratory of Evolutionary Entomology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
  • Turlings TCJ; Laboratory of Fundamental and Applied Research in Chemical Ecology, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
J Pest Sci (2004) ; 96(3): 1061-1075, 2023.
Article em En | MEDLINE | ID: mdl-37181825
ABSTRACT
Cucurbitaceae plants produce cucurbitacins, bitter triterpenoids, to protect themselves against various insects and pathogens. Adult banded cucumber beetles (Diabrotica balteata), a common pest of maize and cucurbits, sequester cucurbitacins, presumably as a defensive mechanism against their natural enemies, which might reduce the efficacy of biological control agents. Whether the larvae also sequester and are protected by cucurbitacins is unclear. We profiled cucurbitacin levels in four varieties of cucumber, Cucumis sativus, and in larvae fed on these varieties. Then, we evaluated larval growth and resistance against common biocontrol organisms including insect predators, entomopathogenic nematodes, fungi and bacteria. We found considerable qualitative and quantitative differences in the cucurbitacin levels of the four cucumber varieties. While two varieties were fully impaired in their production, the other two accumulated high levels of cucurbitacins. We also observed that D. balteata larvae sequester and metabolize cucurbitacins, and although the larvae fed extensively on both belowground and aboveground tissues, the sequestered cucurbitacins were mainly derived from belowground tissues. Cucurbitacins had no detrimental effects on larval performance and, surprisingly, did not provide protection against any of the natural enemies evaluated. Our results show that D. balteata larvae can indeed sequester and transform cucurbitacins, but sequestered cucurbitacins do not impact the biocontrol potential of common natural enemies used in biocontrol. Hence, this plant trait should be conserved in plant breeding programs, as it has been demonstrated in previous studies that it can provide protection against plant pathogens and generalist insects. Supplementary Information The online version contains supplementary material available at 10.1007/s10340-022-01568-3.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Qualitative_research Idioma: En Revista: J Pest Sci (2004) Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Qualitative_research Idioma: En Revista: J Pest Sci (2004) Ano de publicação: 2023 Tipo de documento: Article