Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry.
Methods Mol Biol
; 2674: 251-259, 2023.
Article
em En
| MEDLINE
| ID: mdl-37258973
ABSTRACT
Antibody binding to bacterial surfaces plays a crucial role in immunity, and a key characteristic of this protein-protein interaction is the binding affinity. Determining the affinity of an antibody binding to its antigen is the first step in predicting the function in a physiological environment where other competing protein interactions may be present. Antibody-antigen affinity is often evaluated with isolated proteins. It is informative to also be able to assess antibody binding to a bacterial surface where many antigens might be present, including multiple copies of the specific antigen the antibody recognizes, and in a context where the antigen might be in a more natural conformation. In this chapter, we present a flow cytometry-based assay to measure and calculate the cell surface binding affinity or avidity of any mono- or polyclonal antibody solution.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos
/
Antígenos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2023
Tipo de documento:
Article