Your browser doesn't support javascript.
loading
Caged luciferins enable rapid multicomponent bioluminescence imaging.
Navarro, Mariana X; Brennan, Caroline K; Love, Anna C; Prescher, Jennifer A.
Afiliação
  • Navarro MX; Department of Chemistry, University of California, Irvine, Irvine, California, USA.
  • Brennan CK; Department of Chemistry, University of California, Irvine, Irvine, California, USA.
  • Love AC; Department of Chemistry, University of California, Irvine, Irvine, California, USA.
  • Prescher JA; Department of Chemistry, University of California, Irvine, Irvine, California, USA.
Photochem Photobiol ; 100(1): 67-74, 2024.
Article em En | MEDLINE | ID: mdl-37259257
Bioluminescence is a sensitive technique for imaging biological features over time. Historically, though, the modality has been challenging to employ for multiplexed tracking due to a lack of resolvable luciferase-luciferin pairs. Recent years have seen the development of numerous orthogonal probes for multi-parameter imaging. While successful, generating such tools often requires complex syntheses and lengthy enzyme evolution campaigns. This work showcases an alternative strategy for multiplexed bioluminescence that takes advantage of already-orthogonal caged luciferins and established uncaging enzymes. These probes generate unique bioluminescent signals that can be distinguished via a linear unmixing algorithm. Caged luciferins enabled two- and three-component imaging on the minutes time scale. We further showed that the tools can be used in conjunction with endogenous enzymes for multiplexed studies. Collectively, this approach lowers the barrier to multicomponent bioluminescence imaging and can be readily adopted by the broader community.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferinas / Medições Luminescentes Idioma: En Revista: Photochem Photobiol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Luciferinas / Medições Luminescentes Idioma: En Revista: Photochem Photobiol Ano de publicação: 2024 Tipo de documento: Article