Linoleic acid hydroperoxide increases the transfer of albumin across cultured endothelial monolayers.

ABSTRACT

Exposure to albumin-bound linoleic acid (60 to 150 microM) for 24 h significantly increased the rate of albumin transfer across cultured endothelial monolayers. The increase was dependent on the linoleic acid (182) concentration to which the cultures were exposed. Linoleic acid hydroperoxide (182-OOH) further accelerated the rate of albumin transfer over that of 182. A near maximum albumin transfer was observed after a 2-h incubation with 90 microM 182-OOH. Cells exposed to 182-OOH caused a marked release of lactate dehydrogenase into the media. On the other hand, 182 at concentrations as high as 150 microM, did not significantly affect lactate dehydrogenase release. These results suggest that exposure to 182, and in particular to 182-OOH, reduces the ability of the endothelium to act as a selective permeability barrier to plasma components.