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Enhanced acetate utilization for value-added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis.
Huang, Congcong; Chen, Yaru; Cheng, Shuai; Li, Mengxu; Wang, Luxin; Cheng, Meijie; Li, Feng; Cao, Yingxiu; Song, Hao.
Afiliação
  • Huang C; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
  • Chen Y; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.
  • Cheng S; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
  • Li M; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.
  • Wang L; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
  • Cheng M; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.
  • Li F; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
  • Cao Y; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, China.
  • Song H; Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Biotechnol Bioeng ; 120(10): 3013-3024, 2023 10.
Article em En | MEDLINE | ID: mdl-37306471
The limited supply of reducing power restricts the efficient utilization of acetate in Yarrowia lipolytica. Here, microbial electrosynthesis (MES) system, enabling direct conversion of inward electrons to NAD(P)H, was used to improve the production of fatty alcohols from acetate based on pathway engineering. First, the conversion efficiency of acetate to acetyl-CoA was reinforced by heterogenous expression of ackA-pta genes. Second, a small amount of glucose was used as cosubstrate to activate the pentose phosphate pathway and promote intracellular reducing cofactors synthesis. Third, through the employment of MES system, the final fatty alcohols production of the engineered strain YLFL-11 reached 83.8 mg/g dry cell weight (DCW), which was 6.17-fold higher than the initial production of YLFL-2 in shake flask. Furthermore, these strategies were also applied for the elevation of lupeol and betulinic acid synthesis from acetate in Y. lipolytica, demonstrating that our work provides a practical solution for cofactor supply and the assimilation of inferior carbon sources.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Yarrowia / Engenharia Metabólica Idioma: En Revista: Biotechnol Bioeng Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Yarrowia / Engenharia Metabólica Idioma: En Revista: Biotechnol Bioeng Ano de publicação: 2023 Tipo de documento: Article