The stability of C-peptide and insulin in plasma and serum samples under different storage conditions.
Clin Chem Lab Med
; 61(12): 2150-2158, 2023 11 27.
Article
em En
| MEDLINE
| ID: mdl-37409980
ABSTRACT
OBJECTIVES:
C-peptide and insulin are peptide hormones and their stability is affected by a number of pre-analytical factors. The study aimed to investigate the impact of sample type, storage temperature and time delays before centrifugation and analysis on the stability of C-peptide and insulin.METHODS:
Ten healthy non-diabetic adults in fasting and non-fasting state were enrolled. 40â¯mL of blood was collected from each participant into SST and dipotassium EDTA tubes. Samples were centrifuged immediately or at timed intervals (8, 12, 48 and 72â¯h). After baseline measurements on the Roche Cobas e602 analyzer using electrochemiluminescence immunoassays, aliquots were stored at room temperature (RT), 2-8 and -20⯰C for 4â¯h to 30â¯days. The percentage deviation (PD) from baseline was calculated and a change greater than desirable biological variation total error was considered clinically significant.RESULTS:
C-peptide was more stable in separated serum than plasma (PD of -5 vs. -13â¯%) samples stored at 2-8⯰C for 7â¯days and was most unstable at RT when centrifugation was delayed (PD -46â¯% in plasma and -74â¯% in serum after 48â¯h). Insulin was more stable in plasma than in serum under the different storage conditions with a minimum PD of -1% when stored at -20⯰C for 30â¯days. When samples were kept unspun at RT for 72â¯h, PD was -23 and -80â¯% in plasma and serum, respectively.CONCLUSIONS:
C-peptide was more stable in serum provided the sample was centrifuged immediately and stored in the fridge or freezer while insulin was found to be more stable in EDTA plasma.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Insulina
Limite:
Adult
/
Humans
Idioma:
En
Revista:
Clin Chem Lab Med
Ano de publicação:
2023
Tipo de documento:
Article