Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein-Protein Interactions in Living Cells.
Methods Mol Biol
; 2690: 117-120, 2023.
Article
em En
| MEDLINE
| ID: mdl-37450142
ABSTRACT
Bimolecular fluorescence complementation (BiFC) assay is a method to visualize the protein-protein interaction in living cells. This technique is based on ability of the non-fluorescent fragment of fluorescent protein to form fluorescent complex when they are fused to two interacting proteins. In this chapter, we describe the widely used split yellow fluorescent protein (YFP) system to visualize the protein-protein interaction in plant cells.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento de Interação de Proteínas
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2023
Tipo de documento:
Article