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Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar.
Guo, Yayu; Wang, Shufang; Yu, Keji; Wang, Hou-Ling; Xu, Huimin; Song, Chengwei; Zhao, Yuanyuan; Wen, Jialong; Fu, Chunxiang; Li, Yu; Wang, Shuizhong; Zhang, Xi; Zhang, Yan; Cao, Yuan; Shao, Fenjuan; Wang, Xiaohua; Deng, Xin; Chen, Tong; Zhao, Qiao; Li, Lei; Wang, Guodong; Grünhofer, Paul; Schreiber, Lukas; Li, Yue; Song, Guoyong; Dixon, Richard A; Lin, Jinxing.
Afiliação
  • Guo Y; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Wang S; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Yu K; Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
  • Wang HL; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Xu H; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Song C; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Zhao Y; College of Biological Sciences, China Agricultural University, Beijing, 100193, China.
  • Wen J; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Fu C; College of Agriculture, Henan University of Science and Technology, Luoyang, 471003, China.
  • Li Y; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Wang S; Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083, China.
  • Zhang X; Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
  • Zhang Y; Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
  • Cao Y; Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083, China.
  • Shao F; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Wang X; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
  • Deng X; State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China.
  • Chen T; State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China.
  • Zhao Q; Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
  • Li L; Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
  • Wang G; Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
  • Grünhofer P; Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
  • Schreiber L; School of Life Sciences and School of Advanced Agricultural Sciences, Peking University, Beijing, 100871, China.
  • Li Y; National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
  • Song G; Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany.
  • Dixon RA; Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany.
  • Lin J; State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Nat Commun ; 14(1): 4285, 2023 07 18.
Article em En | MEDLINE | ID: mdl-37463897
ABSTRACT
The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Populus / MicroRNAs Idioma: En Revista: Nat Commun Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Populus / MicroRNAs Idioma: En Revista: Nat Commun Ano de publicação: 2023 Tipo de documento: Article