(Pre)analytical considerations concerning the analysis of synovial calprotectin.
Clin Chem Lab Med
; 62(1): 199-206, 2024 01 26.
Article
em En
| MEDLINE
| ID: mdl-37529863
ABSTRACT
OBJECTIVES:
Several studies have demonstrated that synovial calprotectin is a highly accurate biomarker in diagnosing periprosthetic joint infections (PJI). Assuring reliability is of great importance and coincides with adequate preanalytical handling. This study focuses on potentially interfering factors.METHODS:
To assess the stability of synovial calprotectin, the effect of time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination was investigated. In the blood and lipid contamination experiments, hemolyzed and non-hemolyzed blood, homogenized adipose tissue, intralipid and chylomicrons were added. The effect of viscosity was investigated using freeze-thaw cycles, enzymatic pretreatment and sonification.RESULTS:
No effect on synovial calprotectin levels was observed in synovial samples kept at room temperature compared to samples kept at 4⯰C for up to seven days of storage. Freeze-thaw cycles did not result in significantly different calprotectin levels, although samples without EDTA resulted in higher recoveries after 1 and 2 freeze-thaw cycles. Blood and lipid contamination did not interfere with accurate synovial calprotectin analysis. Sample pretreatment to reduce sample viscosity by pretreating samples with DNAse and/or hyaluronidase did not influence calprotectin analysis. Sonification, however, resulted in increased calprotectin values.CONCLUSIONS:
Synovial calprotectin is a stable biomarker and its analysis is not easily influenced by potential interfering factors.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complexo Antígeno L1 Leucocitário
/
Lipídeos
Limite:
Humans
Idioma:
En
Revista:
Clin Chem Lab Med
Ano de publicação:
2024
Tipo de documento:
Article