Interleukin-6 stimulates in vitro development of late-stage ovine embryos.
J Reprod Immunol
; 159: 104133, 2023 09.
Article
em En
| MEDLINE
| ID: mdl-37647796
ABSTRACT
The effect of interleukin-6 (IL-6) supplementation during the different phases of in vitro embryo culturing (IVC) on embryo development and embryonic gene expression was studied in ovine. IL-6 was added to IVC medium during the late phases (72-192â¯h; 5, 10, and 25â¯ng/ml IL-6) or entire period (0-192â¯h; 10â¯ng/ml IL-6) of IVC to determine its effect on embryo development. Further, the effect of IL-6 (10â¯ng/ml) supplementation at the 72â¯h of IVC on gene expressions associated with JAK/STAT signalling and pluripotency in 8-16 cell embryos (1â¯h post-supplementation) and compact morulae (48â¯h post-supplementation), and apoptosis and primitive endoderm (PrE) development in compact morulae was investigated. The supplementation of 10â¯ng/ml IL-6 during the late phases of IVC significantly (Pâ¯<â¯0.05) increased blastocyst formation (35.2⯱â¯1.52%) compared to the control (21.1⯱â¯1.11%), and 5â¯ng/ml (25.9⯱â¯2.98%) or 25â¯ng/ml (16.5⯱â¯0.73%) IL-6 groups. Conversely, IL-6 (10â¯ng/ml) treatment throughout the IVC period significantly (Pâ¯<â¯0.05) decreased the rate of cleavage (55.4⯱â¯1.57%) and blastocyst formation (14.5⯱â¯1.28%) compared to the control group (65.8⯱â¯1.35% and 21.5⯱â¯0.97%, respectively). In 8-16 cell embryos and compact morulae, the IL-6 treatment significantly (Pâ¯<â¯0.05) affected the expression of genes associated with JAK/STAT signalling and pluripotency. Further, the treatment significantly (Pâ¯<â¯0.05) downregulated BAX and CASP3, and upregulated GATA6 expression in compact morulae. In conclusion, IL-6 supplementation affected the in vitro development of ovine embryos in a dose- and time-dependent manner. The beneficial effect of IL-6 on the development of late-stage embryos was mediated through the changes in gene expressions associated with JAK/STAT signalling, pluripotency, apoptosis and PrE development.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interleucina-6
/
Apoptose
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Reprod Immunol
Ano de publicação:
2023
Tipo de documento:
Article