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Crowding alters F-actin secondary structure and hydration.
Chen, Xiaobing; Roeters, Steven J; Cavanna, Francis; Alvarado, José; Baiz, Carlos R.
Afiliação
  • Chen X; Department of Chemistry, University of Texas at Austin, Austin, TX, USA.
  • Roeters SJ; Department of Chemistry, Aarhus University, Aarhus, Denmark.
  • Cavanna F; Department of Anatomy and Neurosciences, Vrije Universiteit, Amsterdam UMC, Amsterdam, Netherlands.
  • Alvarado J; Department of Physics, University of Texas at Austin, Center for Nonlinear Dynamics, Austin, TX, USA.
  • Baiz CR; Department of Physics, University of Texas at Austin, Center for Nonlinear Dynamics, Austin, TX, USA.
Commun Biol ; 6(1): 900, 2023 09 02.
Article em En | MEDLINE | ID: mdl-37660224
Actin, an important component of eukaryotic cell cytoskeleton, regulates cell shape and transport. The morphology and biochemical properties of actin filaments are determined by their structure and protein-protein contacts. Crowded environments can organize filaments into bundles, but less is known about how they affect F-actin structure. This study used 2D IR spectroscopy and spectral calculations to examine how crowding and bundling impact the secondary structure and local environments in filaments and weakly or strongly bundled networks. The results reveal that bundling induces changes in actin's secondary structure, leading to a decrease in ß-sheet and an increase in loop conformations. Strongly bundled networks exhibit a decrease in backbone solvent exposure, with less perturbed α-helices and nearly "locked" ß-sheets. Similarly, the loops become less hydrated but maintain a dynamic environment. These findings highlight the role of loop structure in actin network morphology and stability under morphology control by PEG.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citoesqueleto de Actina / Actinas Idioma: En Revista: Commun Biol Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citoesqueleto de Actina / Actinas Idioma: En Revista: Commun Biol Ano de publicação: 2023 Tipo de documento: Article