Leukocyte migration inhibition test (LMIT) in systemic lupus erythematosus.
Microbiol Immunol
; 22(12): 785-92, 1978.
Article
em En
| MEDLINE
| ID: mdl-377031
ABSTRACT
A leukocyte migration inhibition test (LMIT) utilizing the agarose gel technique was performed with native DNA as an antigen in ten patients with systemic lupus erythematosus (SLE) and five normal subjects. Irrespective of disease activity, supernatants obtained at different time intervals during lymphocyte culture in eight patients with SLE showed significant alteration of migration, either enhancement or inhibition, of normal leukocytes. However, supernatants in the control experiments produced no significant alteration of migration. Polyacrylamide gel electrophoresis of supernatants obtained from the SLE group revealed that the inhibitory activity was present in the albumin region, whereas the enhancement activity was found in the beta-globulin region. These results indicate that the hitherto employed estimation of the leukocyte migration inhibition test based on the total activity of these two factors is insufficient for accurate evaluation of chemical mediators from sensitized lymphocytes and that the separation of these two factors may be important for a greater understanding of cellular immunity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibição de Migração Celular
/
Leucócitos
/
Lúpus Eritematoso Sistêmico
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Microbiol Immunol
Ano de publicação:
1978
Tipo de documento:
Article