Enhancing the thermostability and catalytic activity of Bacillus subtilis chitosanase by saturation mutagenesis of Lys242.

Guo, Jing; Gao, Wenjun; Zhang, Xuan; Pan, Wenxin; Zhang, Xin; Man, Zaiwei; Cai, Zhiqiang

Guo, Jing; Gao, Wenjun; Zhang, Xuan; Pan, Wenxin; Zhang, Xin; Man, Zaiwei; Cai, Zhiqiang.

Afiliação

Guo J; Laboratory of Applied Microbiology, School of Pharmaceutical, Changzhou University, Changzhou, China.
Gao W; Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, Changzhou University, Changzhou, China.
Zhang X; Laboratory of Applied Microbiology, School of Pharmaceutical, Changzhou University, Changzhou, China.
Pan W; Laboratory of Applied Microbiology, School of Pharmaceutical, Changzhou University, Changzhou, China.
Zhang X; Laboratory of Applied Microbiology, School of Pharmaceutical, Changzhou University, Changzhou, China.
Man Z; Laboratory of Applied Microbiology, School of Pharmaceutical, Changzhou University, Changzhou, China.
Cai Z; Laboratory of Applied Microbiology, School of Pharmaceutical, Changzhou University, Changzhou, China.

Biotechnol J ; 19(1): e2300010, 2024 Jan.

Article em En | MEDLINE | ID: mdl-37705423

Assuntos

Bacillus subtilis; Quitosana; Bacillus subtilis/metabolismo; Simulação de Acoplamento Molecular; Glicosídeo Hidrolases/genética; Glicosídeo Hidrolases/metabolismo; Mutagênese; Hidrogênio; Estabilidade Enzimática

Palavras-chave

Bacillus subtilis; GH46; catalytic activity; chitooligosaccharides; chitosanase; saturation mutagenesis; thermostability

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus subtilis / Quitosana Idioma: En Revista: Biotechnol J Ano de publicação: 2024 Tipo de documento: Article

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