miR-22a targets p62/SQSTM1 to negatively affect autophagy and disease resistance of grass carp (Ctenopharyngodon idella).
Fish Shellfish Immunol
; 142: 109124, 2023 Nov.
Article
em En
| MEDLINE
| ID: mdl-37777097
ABSTRACT
MicroRNAs (miRNAs) are integral to many biological functions, including autophagy, a process recently proven to be closely linked to innate immunity. In this study, we present findings on miR-22a, a teleost homolog of mammalian miR-22, illustrating its capacity to target the autophagy adaptor p62, subsequently inducing downregulation at both mRNA and protein levels. Utilizing Western blot analyses, we demonstrated that miR-22a inhibits the autophagy flux of CIK cells, correlated with an elevated presence of LC3 II. Additionally, the overexpression of miR-22a resulted in the suppression of NF-κB signaling, leading to reduced cellar antimicrobial abilities and increased apoptosis. These findings provide novel insights into the role of miR-22a as an autophagy-related miRNA and its immune mechanisms against pathogens via p62 in teleost, enriching our understanding of the interplay between autophagy and innate immunity.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carpas
/
MicroRNAs
/
Doenças dos Peixes
Limite:
Animals
Idioma:
En
Revista:
Fish Shellfish Immunol
Ano de publicação:
2023
Tipo de documento:
Article