Integrated fermentative process for lipid and ß-carotene production from acetogenic syngas fermentation using an engineered oleaginous Yarrowia lipolytica yeast.

An engineered Yarrowia lipolytica strain was successfully employed to produce ß-carotene and lipids from acetic acid, a product of syngas fermentation by Clostridium aceticum. The strain showed acetic acid tolerance up to concentrations of 20 g/L. Flask experiments yielded a peak lipid content of 33.7 % and ß-carotene concentration of 13.6 mg/g under specific nutrient conditions. The study also investigated pH effects on production in bioreactors, revealing optimal lipid and ß-carotene contents at pH 6.0, reaching 22.9 % and 44 mg/g, respectively. Lipid profiles were consistent across experiments, with C18:1 being the dominant compound at approximately 50 %. This research underscores a green revolution in bioprocessing, showing how biocatalysts can convert syngas, a potentially polluting byproduct, into valuable ß-carotene and lipids with a Y. lipolytica strain.