Invariant BECN1 CXXC motifs bind Zn2+ and regulate structure and function of the BECN1 intrinsically disordered region.

ABSTRACT

ABBREVIATIONS AFM aromatic finger mutant; BH3D BCL2 homology 3 domain; CCD coiled-coil domain; CD circular dichroism spectroscopy; [CysDM1] C18S and C21S double mutant; [CysDM2] C137S, and C140S double mutant; [CysTM], C18S, C21S, C137S, and C140S tetrad mutant; Dmax maximum particle diameter; dRI, differential refractive index; EFA evolving factor analysis; FHD flexible helical domain; FL full length; GFP green fluorescent protein; HDX-MS hydrogen/deuterium exchange mass spectrometry; ICP-MS inductively coupled plasma mass spectrometry; IDR intrinsically disordered region; ITC, isothermal titration calorimetry; MALS, multi angle light scattering; MBP maltose-binding protein; MoRFs molecular recognition features; P(r) pairwise-distance distribution; PtdIns3K class III phosphatidylinositol 3-kinase; Rg radius of gyration; SASBDB small angle scattering biological data bank; SEC size-exclusion chromatography; SEC-SAXS size-exclusion chromatography in tandem with small angle X-ray scattering; TEV tobacco-etch virus; TFE 2,2,2-trifluoroethanol; TPEN N,N,N,N-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine; Vc volume of correlation; WT wild-type.