Psychroserpens ponticola sp. nov. and Marinomonas maritima sp. nov., isolated from seawater.
Int J Syst Evol Microbiol
; 73(10)2023 Oct.
Article
em En
| MEDLINE
| ID: mdl-37830909
ABSTRACT
Two Gram-stain-negative, catalase- and oxidase-positive, aerobic non-motile and motile rod bacteria, strains MSW6T and RSW2T, were isolated from surface seawater. Strain MSW6T optimally grew at 20â°C, pH 7.0 and 3â% NaCl, while strain RSW2T optimally grew at 25â°C, pH 7.0-8.0 and 2â% NaCl. Strain MSW6T possessed menaquinone-6 as the major respiratory quinone, and its major fatty acids were iso-C15ââ1 G, iso-C15ââ0 and iso-C15ââ0 3-OH. The major polar lipid identified in strain MSW6T was phosphatidylethanolamine (PE). On the other hand, strain RSW2T had ubiquinone-8 as the predominant respiratory quinone, and its major fatty acids consisted of summed feature 3 (C16ââ1 ω7c and/or C16ââ1 ω6c), summed feature 8 (C18ââ1 ω7c and/or C18ââ1 ω6c) and C16ââ0. The major polar lipids identified in strain RSW2T were PE and phosphatidylglycerol. As the sole respiratory quinone, strain MSW6T possessed menaquinone-6, while strain RSW2T had ubiquinone-8. The DNA G+C contents of strains MSW6T and RSW2T were 31.9 and 43.4âmol%, respectively. Phylogenetic analyses based on 16S rRNA and core gene sequences showed that strain MSW6T formed a phylogenic lineage with Psychroserpens mesophilus KOPRI 13649T, while strain RSW2T formed a phylogenic lineage with Marinomonas primoryensis KMM 3633T. Strain MSW6T shared 97.9â% 16S rRNA gene sequence similarity and 80.7â% average nucleotide identity (ANI) ith P. mesophilus KOPRI 13649T, and strain RSW2T shared 99.1â% 16S rRNA gene sequence similarity and 93.1â% ANI with M. primoryensis KMM 3633T. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strains MSW6T and RSW2T represent novel species of the genera Psychroserpens and Marinomonas, respectively, for which the names Psychroserpens ponticola sp. nov. and Marinomonas maritima sp. nov. are proposed, respectively. The type strain of P. ponticola is MSW6T (=KACC 22338T=JCM 35022T) and the type strain of M. maritima is RSW2T (=KACC 22716T=JCM 35550T).
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Marinomonas
/
Ácidos Graxos
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Ano de publicação:
2023
Tipo de documento:
Article