Sample preparation and analysis protocols for the elucidation of structure and chemical distribution in kidney stones.

ABSTRACT

Examining intact kidney stones both qualitatively and quantitatively can be difficult due to their size and fragility. Many modern analysis methods often lead to the destruction of the stone's structure during sample preparation. Preserving the structural integrity is crucial for accurately determining the chemical distribution of the components of kidney stones, which, in turn, improves our understanding of the disease's etiology. Infrared microspectroscopy and imaging play a vital role in revealing the stone's microstructure and component distribution. Consequently, this research focuses on investigating the impact of different sample preparation techniques on kidney stone analysis using infrared microspectroscopy. Specifically, it explores how polishing the surface of cross-sectioned stones influences the results. The polishing was performed utilizing abrasive discs and lapping films. A polishing device was also designed for the optimization of sample preparation. Additionally, this work involved a comparison of reflection infrared imaging with Attenuated Total Internal Reflection (ATR) infrared microspectroscopic imaging for the analysis of the microstructure of urinary stones.