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An isothermal CRISPR-based diagnostic assay for Neisseria gonorrhoeae and Chlamydia trachomatis detection.
Luo, Hao; Zeng, Lihong; Yin, Xiaona; Pan, Yuying; Yang, Jianjiang; Liu, Mingjing; Qin, Xiaolin; Feng, Zhanqin; Chen, Wentao; Zheng, Heping.
Afiliação
  • Luo H; Dermatology Hosptial, Southern Medical University , Guangzhou, China.
  • Zeng L; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control , Guangzhou, China.
  • Yin X; Dermatology Hosptial, Southern Medical University , Guangzhou, China.
  • Pan Y; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control , Guangzhou, China.
  • Yang J; Dermatology Hosptial, Southern Medical University , Guangzhou, China.
  • Liu M; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control , Guangzhou, China.
  • Qin X; Dermatology Hosptial, Southern Medical University , Guangzhou, China.
  • Feng Z; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control , Guangzhou, China.
  • Chen W; Dermatology Hosptial, Southern Medical University , Guangzhou, China.
  • Zheng H; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control , Guangzhou, China.
Microbiol Spectr ; 11(6): e0046423, 2023 Dec 12.
Article em En | MEDLINE | ID: mdl-37882532
IMPORTANCE: A method for Neisseria gonorrhoeae (NG)/Chlamydia trachomatis (CT) detection is developed using multiplex-recombinase polymerase amplification and Cas12a/Cas13a. This method can detect NG and CT simultaneously with high sensitivity and specificity. This method has great potential to be further developed into larger-scale screening and point-of-care testing (POCT).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Chlamydia / Gonorreia Limite: Humans Idioma: En Revista: Microbiol Spectr Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Chlamydia / Gonorreia Limite: Humans Idioma: En Revista: Microbiol Spectr Ano de publicação: 2023 Tipo de documento: Article