Effect of Sulforaphane on cardiac injury induced by sepsis in a mouse model: Role of toll-like receptor 4.
J Med Life
; 16(7): 1120-1126, 2023 Jul.
Article
em En
| MEDLINE
| ID: mdl-37900081
ABSTRACT
As sepsis is associated with a 50% increase in mortality, sepsis-induced cardiomyopathy has become a critical topic. A multidisciplinary approach is required for the diagnosis and treatment of septic cardiomyopathy. This study looked at Sulforaphane, a natural product that aims to evaluate cardiac function after sepsis, and its likely mechanism of action. Twenty-four adult male Swiss albino mice were randomly divided into 4 equal groups (n=6) sham, CLP, vehicle Sulforaphane (the same amount of DMSO injected IP one hour before the CLP), and Sulforaphane group (one hour before the CLP, a 5mg/kg dose of Sulforaphane was injected). Cardiac tissue levels of toll-like receptor 4 (TLR-4), pro-inflammatory mediators, anti-inflammatory markers, oxidative stress markers, apoptosis markers, and serum cardiac damage biomarkers were assessed using ELISA. Statistical analyses, including t-tests and ANOVA tests, were performed with a significance level of 0.05 for normally distributed data. Compared to the sham group, the sepsis group had significantly elevated levels of TLR-4, IL-6, TNF-α, MIF, F2-isoprostane, caspase-3, cTn-I, and CK-MB (p<0.05). In contrast, the Sulforaphane pre-treated group demonstrated significantly lower levels of these markers (p<0.05). Additionally, Bcl-2 levels were significantly reduced (p<0.05) in the Sulforaphane group. Sulforaphane administration also significantly attenuated cardiac tissue injury (p<0.05). The findings suggest that Sulforaphane can decrease heart damage in male mice during CLP-induced polymicrobial sepsis by suppressing TLR-4/NF-kB downstream signal transduction pathways.
Palavras-chave
ANOVA: Analysis of Variance; AP-1: Activator protein 1; Bcl-2: B-cell lymphoma 2; CK-MB: creatinine kinase MB; CLP; CLP: Cecal Ligation And Puncture; DMSO-Dimethyl Sulfoxide; ELISA: enzyme-linked immunosorbent assay; ERK: Extracellular signal-regulated kinase; IL-10: Interleukin 10; IL-6: Interleukin 6; IP: intraperitoneally; JNK: c-Jun N-terminal kinase; KEAP-1: Kelch-like ECH-associated protein 1; LPS: lipopolysaccharide; MAPK: Mitogen-Activated Protein Kinase; MD-2: Myeloid differentiation factor-2; MIF: Macrophage migration inhibitory; NF-κB: Nuclear factor kappa-light-chain-enhancer of activated B cells; Nrf2: nuclear factor erythroid 2-related factor 2; Oxidative stress; ROS: reactive oxygen species; Sulforaphane; TLR- 4/NF- kB signaling pathways; TLR4: Toll-like receptor 4; TNF- α: Tumor necrosis factor alpha; cTn-I: cardiac troponin I; cytokines; sepsis; °C: Celsius Degree
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Sepse
/
Traumatismos Cardíacos
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Cardiomiopatias
Limite:
Animals
Idioma:
En
Revista:
J Med Life
Ano de publicação:
2023
Tipo de documento:
Article