An nhr-85::GFP::AID*::3xFLAG knock-in allele for investigation of molting and oscillatory gene regulation.
MicroPubl Biol
; 20232023.
Article
em En
| MEDLINE
| ID: mdl-37927911
ABSTRACT
C. elegans NHR-85 is a poorly characterized nuclear hormone receptor transcription factor with an emerging role in regulating microRNA expression to control developmental timing. We generated the first NHR-85 translational fusion by knocking a GFPAID*3xFLAG cassette into the endogenous locus to tag all known isoforms. nhr-85GFPAID*3xFLAG animals have wild-type broodsizes and NHR-85GFP peaks in expression at the start of the L4 stage in epithelial cells. NHR-85 is not expressed in the germline, suggesting that while it might cooperate with the NHR-23 transcription factor to control microRNA expression, NHR-23 promotes spermatogenesis independent of NHR-85 . This nhr-85GFPAID*3xFLAG strain will be a valuable resource for studying when and where NHR-85 acts to promote developmental timing.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
MicroPubl Biol
Ano de publicação:
2023
Tipo de documento:
Article