Design, construction, and application of noncanonical redox cofactor infrastructures.
Curr Opin Biotechnol
; 84: 103019, 2023 Dec.
Article
em En
| MEDLINE
| ID: mdl-37939631
ABSTRACT
Controlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function orthogonally to the endogenous cofactors, support pathway thermodynamics optimization, and achieve product scopes previously difficult to reach due to endogenous pathway crosstalk. This review will discuss the development of NRC infrastructures, comprising NRC pools, cofactor reduction sources, and cofactor oxidation sinks, the (pool-source-sink) infrastructure.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
/
NAD
Idioma:
En
Revista:
Curr Opin Biotechnol
Ano de publicação:
2023
Tipo de documento:
Article