VF-Pred: Predicting virulence factor using sequence alignment percentage and ensemble learning models.

ABSTRACT

This study introduces VF-Pred, a novel framework developed for the purpose of detecting virulence factors (VFs) through the analysis of genomic data. VFs are crucial for pathogens to successfully infect host tissue and evade the immune system, leading to the onset of infectious diseases. Identifying VFs accurately is of utmost importance in the quest for developing potent drugs and vaccines to counter these diseases. To accomplish this, VF-Pred combines various feature engineering techniques to generate inputs for distinct machine learning classification models. The collective predictions of these models are then consolidated by a final downstream model using an innovative ensembling approach. One notable aspect of VF-Pred is the inclusion of a novel Seq-Alignment feature, which significantly enhances the accuracy of the employed machine learning algorithms. The framework was meticulously trained on 982 features obtained from extensive feature engineering, utilizing a comprehensive ensemble of 25 models. The new downstream ensembling technique adopted by VF-Pred surpasses existing stacking strategies and other ensembling methods, delivering superior performance in VF detection. There have been similar studies done earlier, VF-Pred stands out in comparison showing higher accuracy (83.5 %), higher sensitivity (87 %) towards identification of VFs. Accessible through a user-friendly web page, VF-Pred can be accessed by providing the identifier and protein sequence, enabling the prediction of high or low likelihoods of VFs. Overall, VF-Pred showcases a highly promising methodology for the identification of VFs, potentially paving the way for the development of more effective strategies in the battle against infectious diseases.