Bioanalysis of six antibiotics from volumetric microsamples: a new tool for precision dosing in critically ill children.
Bioanalysis
; 16(1): 19-31, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-37991215
ABSTRACT
Background:
Volumetric absorptive microsamples (VAMS) can support pharmacokinetic / pharmacodynamic studies. We present the bioanalytical method development for the simultaneous quantification of ampicillin, cefepime, ceftriaxone, meropenem, piperacillin, tazobactam, and vancomycin from VAMS. Methods &results:
Optimal extraction, chromatographic, and mass spectrometry conditions were identified. Maximum extraction recoveries included 100 µl of water for rehydration and methanol for protein precipitation. Chromatographic separation used Phenomenex Kinetex™ Polar C18 column with a mobile phase comprising water/acetonitrile with formic acid and was fully validated. Hematocrit effects were only observed for vancomycin. Samples were stable for 90 days at -80°C except for meropenem, which was stable for 60 days.Conclusion:
Multiple antibiotics can be assayed from a single VAMS sample to facilitate pharmacokinetic/pharmacodynamic studies.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vancomicina
/
Antibacterianos
Limite:
Child
/
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2024
Tipo de documento:
Article