Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules.
Science
; 382(6675): 1184-1190, 2023 12 08.
Article
em En
| MEDLINE
| ID: mdl-38060647
ABSTRACT
Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during cell division. An error correction mechanism drives the turnover of kinetochore-microtubule attachments until biorientation is achieved. The structural basis for how kinetochore-mediated chromosome segregation is accomplished and regulated remains an outstanding question. In this work, we describe the cryo-electron microscopy structure of the budding yeast outer kinetochore Ndc80 and Dam1 ring complexes assembled onto microtubules. Complex assembly occurs through multiple interfaces, and a staple within Dam1 aids ring assembly. Perturbation of key interfaces suppresses yeast viability. Force-rupture assays indicated that this is a consequence of impaired kinetochore-microtubule attachment. The presence of error correction phosphorylation sites at Ndc80-Dam1 ring complex interfaces and the Dam1 staple explains how kinetochore-microtubule attachments are destabilized and reset.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Cinetocoros
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Proteínas de Ciclo Celular
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Proteínas de Saccharomyces cerevisiae
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Proteínas Associadas aos Microtúbulos
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Microtúbulos
Idioma:
En
Revista:
Science
Ano de publicação:
2023
Tipo de documento:
Article