GCLiPP: global crosslinking and protein purification method for constructing high-resolution occupancy maps for RNA binding proteins.
Genome Biol
; 24(1): 281, 2023 Dec 07.
Article
em En
| MEDLINE
| ID: mdl-38062486
GCLiPP is a global RNA interactome capture method that detects RNA-binding protein (RBP) occupancy transcriptome-wide. GCLiPP maps RBP-occupied sites at a higher resolution than phase separation-based techniques. GCLiPP sequence tags correspond with known RBP binding sites and are enriched for sites detected by RBP-specific crosslinking immunoprecipitation (CLIP) for abundant cytosolic RBPs. Comparison of human Jurkat T cells and mouse primary T cells uncovers shared peaks of GCLiPP signal across homologous regions of human and mouse 3' UTRs, including a conserved mRNA-destabilizing cis-regulatory element. GCLiPP signal overlapping with immune-related SNPs uncovers stabilizing cis-regulatory regions in CD5, STAT6, and IKZF1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação a RNA
/
Transcriptoma
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Genome Biol
Ano de publicação:
2023
Tipo de documento:
Article