Your browser doesn't support javascript.
loading
GCLiPP: global crosslinking and protein purification method for constructing high-resolution occupancy maps for RNA binding proteins.
Zhu, Wandi S; Litterman, Adam J; Sekhon, Harshaan S; Kageyama, Robin; Arce, Maya M; Taylor, Kimberly E; Zhao, Wenxue; Criswell, Lindsey A; Zaitlen, Noah; Erle, David J; Ansel, K Mark.
Afiliação
  • Zhu WS; Department of Microbiology & Immunology and Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA, USA.
  • Litterman AJ; Department of Microbiology & Immunology and Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA, USA.
  • Sekhon HS; Department of Microbiology & Immunology and Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA, USA.
  • Kageyama R; University of California Berkeley, Berkeley, CA, USA.
  • Arce MM; Department of Microbiology & Immunology and Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA, USA.
  • Taylor KE; Department of Microbiology & Immunology and Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA, USA.
  • Zhao W; Department of Medicine, University of California San Francisco, San Francisco, USA.
  • Criswell LA; Russell/Engleman Rheumatology Research Center, University of California San Francisco, San Francisco, USA.
  • Zaitlen N; Department of Medicine, University of California San Francisco, San Francisco, USA.
  • Erle DJ; Lung Biology Center, University of California San Francisco, San Francisco, USA.
  • Ansel KM; School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, People's Republic of China.
Genome Biol ; 24(1): 281, 2023 Dec 07.
Article em En | MEDLINE | ID: mdl-38062486
GCLiPP is a global RNA interactome capture method that detects RNA-binding protein (RBP) occupancy transcriptome-wide. GCLiPP maps RBP-occupied sites at a higher resolution than phase separation-based techniques. GCLiPP sequence tags correspond with known RBP binding sites and are enriched for sites detected by RBP-specific crosslinking immunoprecipitation (CLIP) for abundant cytosolic RBPs. Comparison of human Jurkat T cells and mouse primary T cells uncovers shared peaks of GCLiPP signal across homologous regions of human and mouse 3' UTRs, including a conserved mRNA-destabilizing cis-regulatory element. GCLiPP signal overlapping with immune-related SNPs uncovers stabilizing cis-regulatory regions in CD5, STAT6, and IKZF1.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Ligação a RNA / Transcriptoma Limite: Animals / Humans Idioma: En Revista: Genome Biol Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Ligação a RNA / Transcriptoma Limite: Animals / Humans Idioma: En Revista: Genome Biol Ano de publicação: 2023 Tipo de documento: Article