Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and ßcatenin on the proliferation and migration of C6 GSCs induced by lowdose radiation.
Oncol Rep
; 51(2)2024 02.
Article
em En
| MEDLINE
| ID: mdl-38099414
ABSTRACT
The radioresistance of glioma is an important cause of treatment failure and tumor aggressiveness. In the present study, under performed with linear accelerator, the effects of 0.3 and 3.0 Gy lowdose radiation (LDR) on the proliferation and migration of C6 glioma stem cells in vitro were examined by flow cytometric analysis, immunocytochemistry and western blot analysis. It was found that lowdose ionizing radiation (0.3 Gy) stimulated the proliferation and migration of these cells, while 3.0 Gy ionizing radiation inhibited the proliferation of C6 glioma stem cells, which was mediated through enhanced Wnt/ßcatenin signaling, which is associated with glioma tumor aggressiveness. LDR treatment increased the expression of the DNA damage marker γH2AX but promoted cell survival with a significant reduction in apoptotic and necrotic cells. When LDR cells were also treated with an inhibitor of Wnt receptor 1 (IWR1), cell proliferation and migration were significantly reduced. IWR1 treatment significantly inhibited Wnt1, Wnt3a and ßcatenin protein expression. Collectively, the current results demonstrated that IWR1 treatment effectively radiosensitizes glioma stem cells and helps to overcome the survival advantages promoted by LDR, which has significant implications for targeted treatment in radioresistant gliomas.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta Catenina
/
Glioma
Limite:
Humans
Idioma:
En
Revista:
Oncol Rep
Ano de publicação:
2024
Tipo de documento:
Article