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In Vivo Raman Spectroscopy Reveals Biochemical Composition of the Esophageal Tissue in Pediatric Eosinophilic Esophagitis.
Locke, Andrea; Haugen, Ezekiel; Thomas, Giju; Correa, Hernan; Dellon, Evan S; Mahadevan-Jansen, Anita; Hiremath, Girish.
Afiliação
  • Locke A; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA.
  • Haugen E; Vanderbilt Biophotonics Center, Nashville, Tennessee, USA.
  • Thomas G; Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
  • Correa H; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA.
  • Dellon ES; Vanderbilt Biophotonics Center, Nashville, Tennessee, USA.
  • Mahadevan-Jansen A; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA.
  • Hiremath G; Vanderbilt Biophotonics Center, Nashville, Tennessee, USA.
Clin Transl Gastroenterol ; 15(2): e00665, 2024 02 01.
Article em En | MEDLINE | ID: mdl-38112293
ABSTRACT

INTRODUCTION:

Biochemical alterations in the esophagus of patients with eosinophilic esophagitis (EoE) are poorly understood. We used Raman spectroscopy through a pediatric endoscope to identify key Raman features reflective of the esophageal biochemical composition to differentiate between children with EoE from non-EoE controls and between children with active (aEoE) and inactive EoE (iEoE).

METHODS:

Spectral measurements were obtained using a customized pediatric endoscope-compatible fiber-optic Raman probe in real time during an esophagogastroduodenoscopy. Chemometric analysis was performed to identify key Raman features associated with EoE. Pearson correlation analysis was used to assess relationship between the key Raman features and EoE activity indices. Their diagnostic utility was ascertained using the receiver operator characteristic curve analysis.

RESULTS:

Forty-three children were included in the study (EoE = 32 [74%] and non-EoE control = 11 [26%]; aEoE = 20 [63%] and iEoE = 12 [37%]). Raman intensities assigned to lipids, proteins, and glycogenprotein ratio accurately distinguished children with EoE from those without EoE and aEoE from iEoE. They significantly correlated with EoE activity indices. The Raman peak ratio for lipids had 90.6% sensitivity, 100% specificity, and an area under the curve of 0.95 to differentiate children with EoE from non-EoE controls. The glycogenprotein ratio had 70% sensitivity, 91.7% specificity, and an area under the curve of 0.75 to distinguish children with aEoE from iEoE.

DISCUSSION:

Real-time intraendoscopy Raman spectroscopy is an effective method for identifying spectral markers reflective of the esophageal biochemical composition in children with EoE. This technique may aid in the diagnosis and monitoring of EoE and help to elucidate EoE pathogenesis.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enterite / Eosinofilia / Esofagite Eosinofílica / Gastrite Limite: Child / Humans Idioma: En Revista: Clin Transl Gastroenterol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enterite / Eosinofilia / Esofagite Eosinofílica / Gastrite Limite: Child / Humans Idioma: En Revista: Clin Transl Gastroenterol Ano de publicação: 2024 Tipo de documento: Article