Novel diagnostic approach for amoebic liver abscess using cell free (cf) DNA: a prospective study.
Infect Dis (Lond)
; 56(4): 259-267, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38112684
ABSTRACT
BACKGROUND:
Amoebic liver abscess (ALA) is commonly seen in tropical countries and diagnosis of ALA relies mainly on non-specific serological and imaging techniques as well as PCR from pus.OBJECTIVE:
This study evaluated the potential of using cell free DNA (cfDNA) from serum and urine for diagnosing ALA.METHODS:
We prospectively evaluated quantitative PCR (qPCR) for detection of cf DNA in serum and urine sample in all liver abscess patients. The samples were collected from patients reporting to emergency ward of Postgraduate Institute of Medical Education and Research, Chandigarh, India with symptoms suggestive of liver abscess. Real time PCR was done to detect cf DNA in serum and urine by targeting 99-bp unit of small subunit rRNA of Entamoeba histolytica and conventional PCR for pus.RESULTS:
A total 113 samples (serum and urine) and 100 pus samples were analysed. A total of 62 ALA patients were confirmed; with maximum 57 patients detected by qPCR for cfDNA in the serum, 55 patients by PCR on pus aspirate and 50 ALA patients by qPCR for cfDNA in urine sample. Therefore, the sensitivity of qPCR for detection of cf DNA in serum was 91.94% and for urine was 80.65%.CONCLUSION:
A total of 11.2% of ALA patients were diagnosed only through detection of E. histolytica cf DNA in their serum and urine. Detection of cfDNA from serum, urine of ALA has a potential role in future especially for developing countries as it is a rapid, sensitive and patient friendly diagnostic approach.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos Livres
/
Abscesso Hepático Amebiano
Limite:
Humans
Idioma:
En
Revista:
Infect Dis (Lond)
Ano de publicação:
2024
Tipo de documento:
Article