Transcriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection.
Virol J
; 20(1): 303, 2023 12 19.
Article
em En
| MEDLINE
| ID: mdl-38115115
ABSTRACT
BACKGROUND:
Pseudorabies virus (PRV) causes substantial losses in the swine industry worldwide. Attenuated PRV strains with deletions of immunomodulatory genes glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) are candidate vaccines. However, the effects of gE/gI/TK deletions on PRV-host interactions are not well understood.METHODS:
To characterize the impact of gE/gI/TK deletions on host cells, we analyzed and compared the transcriptomes of PK15 cells infected with wild-type PRV (SD2017), PRV with gE/gI/TK deletions (SD2017gE/gI/TK) using RNA-sequencing.RESULTS:
The attenuated SD2017gE/gI/TK strain showed increased expression of inflammatory cytokines and pathways related to immunity compared to wild-type PRV. Cell cycle regulation and metabolic pathways were also perturbed.CONCLUSIONS:
Deletion of immunomodulatory genes altered PRV interactions with host cells and immune responses. This study provides insights into PRV vaccine design.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudorraiva
/
Doenças dos Suínos
/
Herpesvirus Suídeo 1
Limite:
Animals
Idioma:
En
Revista:
Virol J
Ano de publicação:
2023
Tipo de documento:
Article