Imaging of brain clearance pathways via MRI assessment of the glymphatic system.
Aging (Albany NY)
; 15(24): 14945-14956, 2023 12 26.
Article
em En
| MEDLINE
| ID: mdl-38149988
ABSTRACT
Glymphatic clearance dysfunction may play an important role in a variety of neurodegenerative diseases and the progression of ageing. However, in vivo imaging of the glymphatic system is challenging. In this study, we describe an MRI method based on chemical exchange saturation transfer (CEST) of the Angiopep-2 probe to visualize the clearance function of the glymphatic system. We injected rats with Angiopep-2 via the tail vein and performed in vivo MRI at 7 T to track differences in Angiopep-2 signal changes; we then applied the same principles in a bilateral deep cervical lymph node ligation rat model and in ageing rats. We demonstrated the feasibility of Angiopep-2 CEST for visualizing the clearance function of the glymphatic system. Finally, a pathological assessment was performed. Within the model group, the deep cervical lymph node ligation group and the ageing group showed higher CEST signal than the control group. We conclude that this new MRI method can visualize clearance in the glymphatic system.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasos Linfáticos
/
Sistema Glinfático
Limite:
Animals
Idioma:
En
Revista:
Aging (Albany NY)
/
Aging (Albany, N.Y. Online)
Ano de publicação:
2023
Tipo de documento:
Article