Structural characterization and immunomodulatory effect of a starch-like Grifola frondosa polysaccharides on cyclophosphamide-induced immunosuppression in mice.
Carbohydr Res
; 535: 109011, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-38150753
ABSTRACT
In this study, a pure Grifola frondosa polysaccharide (GFP-1) was extracted and purified from Grifola frondosa. By HPLC, GC-MS, FT-IR, and NMR analysis, GFP-1 was determined to be a starch-like polysaccharide with an average molecular weight of 3370 kDa. It included three monosaccharides, i.e., glucose, galactose, and mannose. The backbone of GFP-1 consisted of â4)-α-Glcp-(1â and â4,6)-α-Glcp-(1 â . The side branches were composed of â6)-α-Galp-(1â, α-Glcp-(1â, and a small amount of α-Manp-(1 â . By using a cyclophosphamide (CTX)-induced immunosuppressed mice model, we evaluated the immunomodulatory activity of GFP-1. The results showed that GFP-1 increased the thymic and spleen indices, promoted the level of IgG and IgA in serum, and activated the mitogen-activated protein kinase (MAPK) pathway in CTX-induced mice. Also, GFP-1 significantly promoted the mRNA expression of intestinal barrier factors and protected intestinal structural integrity in immunosuppressed mice. In conclusion, the data presented here suggested that GFP-1 might be a potential immune-enhancing supplement.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Amido
/
Grifola
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2024
Tipo de documento:
Article