Evaluation of the anti-androgenic and cytotoxic effects of benzophenone-3 in male Sprague-Dawley rats.
J Toxicol Environ Health A
; 87(6): 266-273, 2024 03 18.
Article
em En
| MEDLINE
| ID: mdl-38166509
ABSTRACT
Benzophenone-3 (BP-3, 2-hydroxy-4-methoxybenzophenone, oxybenzone) is one of the most widely used types of benzophenone organic sunscreen. However, this compound is a potentially harmful toxicant. The aim of this study was 2-fold to (1) utilize a Hershberger bioassay in vivo in castrated male Sprague-Dawley rats to investigate the anti-androgenic activities of BP-3, and (2) use in vitro a methyl tetrazolium assay to compare the toxicity between Leydig cells (TM3 cells) and mouse fibroblast (NIH-3T3) cell lines. In the Hershberger assay, rats were divided into 6 groups (each of n = 7) a vehicle control, negative control, positive control, PB-3 low (40 mg/kg), BP-3 intermediate (200 mg/kg), and BP-3 high (1000 mg/kg)-dose. The weight of the ventral prostate was significantly decreased at BP-3 doses of 200 or 1,000 mg/kg/day. In addition, the levator anibulbocavernosus muscle weights were also significantly reduced at BP-3 doses of 40, 200, or 1,000 mg/kg/day. In the MTT assay, the viability of NIH-3T3 mouse fibroblast cells was within the normal range. However, the TM3 mouse testis Leydig cell viability was significantly lowered in a concentration-dependent manner. Therefore, data indicate that BP-3 might exert in vivo anti-androgenic and in vitro cytotoxic effects in cells associated with the male reproductive system compared to normal non-reproductive cells.Abbreviation BP-3 benzophenone-3; CG Cowper's gland; DMEM Dulbecco's modified Eagle's medium; DMSO dimethyl sulfoxide; GP glans penis; LABC levator anibulbocavernosus muscle; MTT methyl tetrazolium; NC negative control; PC positive control; SV seminal vesicle; TP testosterone propionate; VC vehicle control; VP ventral prostate.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Orquiectomia
/
Antineoplásicos
Limite:
Animals
Idioma:
En
Revista:
J Toxicol Environ Health A
Ano de publicação:
2024
Tipo de documento:
Article