Pyrococcus furiosus Argonaute-mediated porcine epidemic diarrhea virus detection.
Appl Microbiol Biotechnol
; 108(1): 137, 2024 Dec.
Article
em En
| MEDLINE
| ID: mdl-38229331
ABSTRACT
Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, induces severe vomiting and acute watery diarrhea in unweaned piglets. The pig industry has suffered tremendous financial losses due to the high mortality rate of piglets caused by PEDV. Consequently, a simple and rapid on-site diagnostic technology is crucial for preventing and controlling PEDV. This study established a detection method for PEDV using recombinase-aided amplification (RAA) and Pyrococcus furiosus Argonaute (PfAgo), which can detect 100 copies of PEDV without cross-reactivity with other pathogens. The entire reaction of RAA and PfAgo to detect PEDV does not require sophisticated instruments, and the reaction results can be observed with the naked eye. Overall, this integrated RAA-PfAgo cleavage assay is a practical tool for accurately and quickly detecting PEDV. KEY POINTS ⢠PfAgo has the potential to serve as a viable molecular diagnostic tool for the detection and diagnosis of viral genomes ⢠The RAA-PfAgo detection technique has a remarkable level of sensitivity and specificity ⢠The RAA-PfAgo detection system can identify PEDV without needing advanced equipment.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
4_TD
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Infecções por Coronavirus
/
Coronavirus
/
Pyrococcus furiosus
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Vírus da Diarreia Epidêmica Suína
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2024
Tipo de documento:
Article