Saliva direct PCR protocol for HLA-DQB1*02 genotyping.
Int J Immunogenet
; 51(2): 89-92, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38296826
ABSTRACT
Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Doença Celíaca
Tipo de estudo:
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
Int J Immunogenet
Ano de publicação:
2024
Tipo de documento:
Article