Protocol to retrieve unknown flanking DNA sequences using semi-site-specific PCR-based genome walking.
STAR Protoc
; 5(1): 102864, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38308839
ABSTRACT
Here, we describe a protocol based on semi-site-specific primer PCR (3SP-PCR) to access unknown flanking DNA sequences. We specify the guidelines for designing primers for 3SP-PCR. We also describe experimental procedures for the 3SP-PCR, along with PCR product purification and subsequent sequencing and analysis. For complete details on the use and execution of this protocol, please refer to Wei et al.1.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article