Development of a live-attenuated chimeric vaccine against the emerging Usutu virus.
Vaccine
; 42(6): 1363-1371, 2024 Feb 27.
Article
em En
| MEDLINE
| ID: mdl-38310016
ABSTRACT
Usutu virus (USUV) is an emerging arthropod-borne flavivirus that has expanded into multiple European countries during the past several decades. USUV infection in human has been linked to severe neurological complications, and no vaccine is now available against USUV. In this work, we develop a live-attenuated chimeric USUV vaccine (termed ChinUSUV) based on the full-length infectious cDNA clone of the licensed Japanese encephalitis virus (JEV) vaccine strain SA14-14-2. In vitro studies demonstrate that ChinUSUV replicates efficiently and maintains its genetic stability. Remarkably, ChinUSUV exhibits a significant attenuation phenotype in multiple mouse models even compared with the licensed JEV vaccine. A single immunization with ChinUSUV elicits potent IgG and neutralizing antibody responses as well as T cell response. Passive transfer of sera from ChinUSUV-immunized mice confers significant protection against lethal homologous challenge in suckling mice. Taken together, our results suggest that ChinUSUV represents a potential USUV vaccine candidate that merits further development.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
1_ASSA2030
Base de dados:
MEDLINE
Assunto principal:
Encefalite Japonesa
/
Vacinas contra Encefalite Japonesa
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Vírus da Encefalite Japonesa (Espécie)
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Flavivirus
Limite:
Animals
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Humans
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2024
Tipo de documento:
Article