Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches.
Methods Mol Biol
; 2762: 251-266, 2024.
Article
em En
| MEDLINE
| ID: mdl-38315370
ABSTRACT
Targeted mass spectrometric analysis is widely employed across various omics fields as a validation strategy due to its high sensitivity and accuracy. The approach has been successfully employed for the structural analysis of proteins, glycans, lipids, and metabolites. Multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) have been the methods of choice for targeted structural studies of biomolecules. These target analyses simplify the analytical workflow, reduce background interference, and increase selectivity/specificity, allowing for a reliable quantification of permethylated N-glycans in complex biological matrices.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2024
Tipo de documento:
Article