pyHiM: a new open-source, multi-platform software package for spatial genomics based on multiplexed DNA-FISH imaging.
Genome Biol
; 25(1): 47, 2024 02 13.
Article
em En
| MEDLINE
| ID: mdl-38351149
ABSTRACT
Genome-wide ensemble sequencing methods improved our understanding of chromatin organization in eukaryotes but lack the ability to capture single-cell heterogeneity and spatial organization. To overcome these limitations, new imaging-based methods have emerged, giving rise to the field of spatial genomics. Here, we present pyHiM, a user-friendly python toolbox specifically designed for the analysis of multiplexed DNA-FISH data and the reconstruction of chromatin traces in individual cells. pyHiM employs a modular architecture, allowing independent execution of analysis steps and customization according to sample specificity and computing resources. pyHiM aims to facilitate the democratization and standardization of spatial genomics analysis.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Genômica
Idioma:
En
Revista:
Genome Biol
Ano de publicação:
2024
Tipo de documento:
Article