New rabies viral resources for multi-scale neural circuit mapping.

Bouin, Alexis; Wu, Ginny; Koyuncu, Orkide O; Ye, Qiao; Kim, Keun-Young; Wu, Michele Y; Tong, Liqi; Chen, Lujia; Phan, Sebastien; Mackey, Mason R; Ramachandra, Ranjan; Ellisman, Mark H; Holmes, Todd C; Semler, Bert L; Xu, Xiangmin

Bouin, Alexis; Wu, Ginny; Koyuncu, Orkide O; Ye, Qiao; Kim, Keun-Young; Wu, Michele Y; Tong, Liqi; Chen, Lujia; Phan, Sebastien; Mackey, Mason R; Ramachandra, Ranjan; Ellisman, Mark H; Holmes, Todd C; Semler, Bert L; Xu, Xiangmin.

Afiliação

Bouin A; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA, 92697, USA.
Wu G; Department Anatomy & Neurobiology, School of Medicine, University of California, Irvine, CA, 92697, USA.
Koyuncu OO; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA, 92697, USA.
Ye Q; Department Anatomy & Neurobiology, School of Medicine, University of California, Irvine, CA, 92697, USA.
Kim KY; Department Biomedical Engineering, University of California, Irvine, CA, 92697, USA.
Wu MY; The National Center for Microscopy and Imaging Research (NCMIR) and the Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Tong L; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, CA, 92697, USA.
Chen L; Department Anatomy & Neurobiology, School of Medicine, University of California, Irvine, CA, 92697, USA.
Phan S; Department Anatomy & Neurobiology, School of Medicine, University of California, Irvine, CA, 92697, USA.
Mackey MR; Department Biomedical Engineering, University of California, Irvine, CA, 92697, USA.
Ramachandra R; The National Center for Microscopy and Imaging Research (NCMIR) and the Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Ellisman MH; The National Center for Microscopy and Imaging Research (NCMIR) and the Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Holmes TC; The National Center for Microscopy and Imaging Research (NCMIR) and the Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Semler BL; The National Center for Microscopy and Imaging Research (NCMIR) and the Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA.
Xu X; Physiology & Biophysics, School of Medicine, University of California, Irvine, CA, 92697, USA.

Mol Psychiatry ; 2024 Feb 14.

Article em En | MEDLINE | ID: mdl-38355784

ABSTRACT

ABSTRACT

Comparisons and linkage between multiple imaging scales are essential for neural circuit connectomics. Here, we report 20 new recombinant rabies virus (RV) vectors that we have developed for multi-scale and multi-modal neural circuit mapping tools. Our new RV tools for mesoscale imaging express a range of improved fluorescent proteins. Further refinements target specific neuronal subcellular locations of interest. We demonstrate the discovery power of these new tools including the detection of detailed microstructural changes of rabies-labeled neurons in aging and Alzheimer's disease mouse models, live imaging of neuronal activities using calcium indicators, and automated measurement of infected neurons. RVs that encode GFP and ferritin as electron microscopy (EM) and fluorescence microscopy reporters are used for dual EM and mesoscale imaging. These new viral variants significantly expand the scale and power of rabies virus-mediated neural labeling and circuit mapping across multiple imaging scales in health and disease.

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Idioma: En Revista: Mol Psychiatry Ano de publicação: 2024 Tipo de documento: Article

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