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1H NMR metabolomics insights into comparative diabesity in male and female zebrafish and the antidiabetic activity of DL-limonene.
Benchoula, Khaled; Serpell, Christopher J; Mediani, Ahmed; Albogami, Abdulaziz; Misnan, Norazlan Mohmad; Ismail, Nor Hadiani; Parhar, Ishwar S; Ogawa, Satoshi; Hwa, Wong Eng.
Afiliação
  • Benchoula K; School of Medicine, Faculty of Health and Medical Sciences, Taylor's University, 1, Jalan Taylors, 47500, Subang Jaya, Selangor, Malaysia.
  • Serpell CJ; School of Pharmacy, University College London, London, WC1N 1AX, UK. chris.serpell@ucl.ac.uk.
  • Mediani A; Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia.
  • Albogami A; Biology Department, Faculty of Science, Al-Baha University, 65779-7738, Alaqiq, Saudi Arabia.
  • Misnan NM; Institute for Medical Research Malaysia, No.1, Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, 40170, Shah Alam, Selangor Darul Ehsan, Malaysia.
  • Ismail NH; Atta-ur-Rahman Institute for Natural Products Discovery, UiTM Puncak Alam Campus, 42300, Puncak Alam, Selangor, Malaysia.
  • Parhar IS; Monash University (Malaysia) BRIMS, Jeffrey Cheah School of Medicine and Health Sciences, Jalan Lagoon Selatan, Bandar Sunway, 47500, Subang Jaya, Selangor, Malaysia.
  • Ogawa S; Monash University (Malaysia) BRIMS, Jeffrey Cheah School of Medicine and Health Sciences, Jalan Lagoon Selatan, Bandar Sunway, 47500, Subang Jaya, Selangor, Malaysia.
  • Hwa WE; School of Medicine, Faculty of Health and Medical Sciences, Taylor's University, 1, Jalan Taylors, 47500, Subang Jaya, Selangor, Malaysia. EngHwa.Wong@taylors.edu.my.
Sci Rep ; 14(1): 3823, 2024 02 15.
Article em En | MEDLINE | ID: mdl-38360784
ABSTRACT
Zebrafish have been utilized for many years as a model animal for pharmacological studies on diabetes and obesity. High-fat diet (HFD), streptozotocin and alloxan injection, and glucose immersion have all been used to induce diabetes and obesity in zebrafish. Currently, studies commonly used both male and female zebrafish, which may influence the outcomes since male and female zebrafish are biologically different. This study was designed to investigate the difference between the metabolites of male and female diabetic zebrafish, using limonene - a natural product which has shown several promising results in vitro and in vivo in treating diabetes and obesity-and provide new insights into how endogenous metabolites change following limonene treatment. Using HFD-fed male and female zebrafish, we were able to develop an animal model of T2D and identify several endogenous metabolites that might be used as diagnostic biomarkers for diabetes. The endogenous metabolites in males and females were different, even though both genders had high blood glucose levels and a high BMI. Treatment with limonene prevented high blood glucose levels and improved in diabesity zebrafish by limonene, through reversal of the metabolic changes caused by HFD in both genders. In addition, limonene was able to reverse the elevated expression of AKT during HFD.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diabetes Mellitus / Hiperglicemia Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diabetes Mellitus / Hiperglicemia Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article