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Polygonum cillinerve polysaccharide inhibits transmissible gastroenteritis virus by regulating microRNA-181.
Duan, Xueqin; Li, Huicong; Tan, Xuewen; Liu, Nishang; Wang, Xingchen; Zhang, Weimin; Liu, Yingqiu; Ma, Wuren; Wu, Yi; Ma, Lin; Fan, Yunpeng.
Afiliação
  • Duan X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China; Agricultural Management Department, Sichuan Xuanhan Vocational Secondary School, Xuanhan 636350, PR
  • Li H; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Tan X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Liu N; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Wang X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Zhang W; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Liu Y; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Ma W; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China.
  • Wu Y; Nanjing Agricultural University, No 1 Weigang, Nanjing 210095, PR China. Electronic address: wuyi2001cn@163.com.
  • Ma L; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China. Electronic address: linma@nwafu.edu.cn.
  • Fan Y; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China; Institute of Traditional Chinese Veterinary Medicine, Northwest A&F University , Yangling 712100, PR China. Electronic address: ypfan@nwsuaf.edu.cn.
Vet J ; 304: 106083, 2024 04.
Article em En | MEDLINE | ID: mdl-38365083
ABSTRACT
Transmissible gastroenteritis virus (TGEV) is an important pathogen capable of altering the expression profile of cellular miRNA. In this study, the potential of Polygonum cillinerve polysaccharide (PCP) to treat TGEV-infected piglets was evaluated through in vivo experiments. High-throughput sequencing technology was employed to identify 9 up-regulated and 17 down-regulated miRNAs during PCP-mediated inhibition of TGEV infection in PK15 cells. Additionally, miR-181 was found to be associated with target genes of key proteins in the apoptosis pathway. PK15 cells were treated with various concentrations of PCP following transfection with miR-181 mimic or inhibitor. Real-time PCR assessed the impact on TGEV replication, while electron microscopy (TEM) and Hoechst fluorescence staining evaluated cellular functionality. Western blot analysis was utilized to assess the expression of key signaling factors-cytochrome C (cyt C), caspase 9, and P53-in the apoptotic signaling pathway. The results showed that compared with the control group, 250 µg/mL PCP significantly inhibited TGEV gRNA replication and gene N expression (P < 0.01). Microscopic examination revealed uniform cell morphology and fewer floating cells in PCP-treated groups (250 and 125 µg/mL). TEM analysis showed no typical virus structure in the 250 µg/mL PCP group, and apoptosis staining indicated a significant reduction in apoptotic cells at this concentration. Furthermore, PCP may inhibit TGEV-induced apoptosis via the Caspase-dependent mitochondrial pathway following miR-181 transfection. These findings provide a theoretical basis for further exploration into the mechanism of PCP's anti-TGEV properties.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Vírus da Gastroenterite Transmissível / Polygonum / MicroRNAs Limite: Animals Idioma: En Revista: Vet J Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Vírus da Gastroenterite Transmissível / Polygonum / MicroRNAs Limite: Animals Idioma: En Revista: Vet J Ano de publicação: 2024 Tipo de documento: Article