Time-dependent changes in retinoids content in liver and adipose tissue after feeding of a vitamin A-deficient diet to mice.
Exp Anim
; 73(3): 302-309, 2024 Jul 09.
Article
em En
| MEDLINE
| ID: mdl-38382988
ABSTRACT
Vitamin A is an important nutrient for multiple physiological functions. To elucidate the role of vitamin A in vivo, vitamin A-deficient diets have been often used in mice to establish a vitamin A-deficiency model. However, the information on the appropriate feeding periods and time course of changes in vitamin A content in organs after the start of vitamin A-deficient diet feeding is lacking. This study aimed to assess the retinoids levels in liver and white adipose tissue in mice fed a vitamin A-deficient diet for ≤8 weeks. High-performance liquid chromatography was used to measure the retinoids levels in liver and white adipose tissue every 2 weeks for ≤8 weeks. Vitamin A-deficient diet feeding significantly decreased retinol in the liver over 6 weeks, but retinyl palmitate, a main storage form of vitamin A, was not changed over 8 weeks. The plasma retinol level remained constant throughout the experiment. In white adipose tissue, retinyl palmitate gradually decreased over 8 weeks. These results indicate that vitamin A-deficient diet feeding longer than 6 weeks reduced retinol in liver and retinyl palmitate in white adipose tissue over 8 weeks, although it is not enough for the induction of a whole-body vitamin A deficiency.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retinoides
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Vitamina A
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Deficiência de Vitamina A
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Dieta
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Diterpenos
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Tecido Adiposo Branco
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Ésteres de Retinil
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Fígado
Limite:
Animals
Idioma:
En
Revista:
Exp Anim
Ano de publicação:
2024
Tipo de documento:
Article