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Synapsin 2a tetramerisation selectively controls the presynaptic nanoscale organisation of reserve synaptic vesicles.
Longfield, Shanley F; Gormal, Rachel S; Feller, Matis; Parutto, Pierre; Reingruber, Jürgen; Wallis, Tristan P; Joensuu, Merja; Augustine, George J; Martínez-Mármol, Ramón; Holcman, David; Meunier, Frédéric A.
Afiliação
  • Longfield SF; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Gormal RS; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Feller M; Group of Data Modelling and Computational Biology, IBENS, Ecole Normale Superieure, 75005, Paris, France.
  • Parutto P; Group of Data Modelling and Computational Biology, IBENS, Ecole Normale Superieure, 75005, Paris, France.
  • Reingruber J; Group of Data Modelling and Computational Biology, IBENS, Ecole Normale Superieure, 75005, Paris, France.
  • Wallis TP; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Joensuu M; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Augustine GJ; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Martínez-Mármol R; Temasek Lifesciences Laboratory, Singapore, Singapore.
  • Holcman D; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4072, Australia.
  • Meunier FA; Group of Data Modelling and Computational Biology, IBENS, Ecole Normale Superieure, 75005, Paris, France.
Nat Commun ; 15(1): 2217, 2024 Mar 12.
Article em En | MEDLINE | ID: mdl-38472171
ABSTRACT
Neurotransmitter release relies on the regulated fusion of synaptic vesicles (SVs) that are tightly packed within the presynaptic bouton of neurons. The mechanism by which SVs are clustered at the presynapse, while preserving their ability to dynamically recycle to support neuronal communication, remains unknown. Synapsin 2a (Syn2a) tetramerization has been suggested as a potential clustering mechanism. Here, we used Dual-pulse sub-diffractional Tracking of Internalised Molecules (DsdTIM) to simultaneously track single SVs from the recycling and the reserve pools, in live hippocampal neurons. The reserve pool displays a lower presynaptic mobility compared to the recycling pool and is also present in the axons. Triple knockout of Synapsin 1-3 genes (SynTKO) increased the mobility of reserve pool SVs. Re-expression of wild-type Syn2a (Syn2aWT), but not the tetramerization-deficient mutant K337Q (Syn2aK337Q), fully rescued these effects. Single-particle tracking revealed that Syn2aK337QmEos3.1 exhibited altered activity-dependent presynaptic translocation and nanoclustering. Therefore, Syn2a tetramerization controls its own presynaptic nanoclustering and thereby contributes to the dynamic immobilisation of the SV reserve pool.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vesículas Sinápticas / Sinapsinas Idioma: En Revista: Nat Commun Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vesículas Sinápticas / Sinapsinas Idioma: En Revista: Nat Commun Ano de publicação: 2024 Tipo de documento: Article