Impacts of cationic lipid-DNA complexes on immune cells and hematopoietic cells in vivo.
Biomater Sci
; 12(9): 2381-2393, 2024 Apr 30.
Article
em En
| MEDLINE
| ID: mdl-38500446
ABSTRACT
The inability to systemic administration of nanoparticles, particularly cationic nanoparticles, has been a significant barrier to their clinical translation due to toxicity concerns. Understanding the in vivo behavior of cationic lipids is crucial, given their potential impact on critical biological components such as immune cells and hematopoietic stem cells (HSC). These cells are essential for maintaining the body's homeostasis, and their interaction with cationic lipids is a key factor in determining the safety and efficacy of these nanoparticles. In this study, we focused on the cytotoxic effects of cationic lipid/DNA complexes (CLN/DNA). Significantly, we observed that the most substantial cytotoxic effects, including a marked increase in numbers of long-term hematopoietic stem cells (LT-HSC), occurred 24 h post-CLN/DNA treatment in mice. Furthermore, we found that CLN/DNA-induced HSC expansion in bone marrow (BM) led to a notable decrease in the ability to reestablish blood cell production. Our study provides crucial insights into the interaction between cationic lipids and vital cellular components of the immune and hematopoietic systems.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Células-Tronco Hematopoéticas
/
Cátions
/
Lipídeos
Limite:
Animals
Idioma:
En
Revista:
Biomater Sci
Ano de publicação:
2024
Tipo de documento:
Article